The device (1) comprises an inlet element (28) comprising an interface (40) for connecting to an artificial vagina and delimiting an inlet opening (14), a filter delimiting with the inlet element (28) an inlet chamber (41) on the side of the filter turned towards the opening (14) and a collection bag (4) extending between a closed end (8) and an open end (7) in which said inlet element is engaged; said bag and said filter delimiting a collection chamber (42) on the side of the filter turned towards the end (8); by means of which the semen entering through the opening (14) passes through the filter and comes to the collection chamber (42); the filter being a perforated wall (30) having a tubular portion (22) and a bottom portion (15) closing said tubular portion, and having a surface density of orifices (24) that is greater on said bottom portion than on said tubular portion.