The device (1) comprises an inlet element (28) comprising an interface (40) for connecting to an artificial vagina and delimiting an inlet opening (14), a filter associated with the inlet element (28) and delimiting therewith an inlet chamber (41) on the side of a first face of the filter turned towards the opening (14) and a collection bag (4) extending between a closed end (8) and an end (7) having an opening (6) in which said inlet element is engaged; said bag and said filter delimiting a collection chamber (42) on the side of the second face (18) of the filter turned towards the end (8); by means of which semen entering through the opening (14) passes through the filter and is collected in the collection chamber (42); characterized in that the filter is formed by a perforated wall (30) in one piece with the inlet element.