E. Lynn Zechiedrich,Jonathan Fogg,Daniel James Catanese, JR.,Nancy Maizel,Olivier Humbert
申请号：
US14404736
公开号：
US20150376645A1
申请日:
2013.05.30
申请国别(地区):
US
年份:
2015
代理人:
摘要：
In some embodiments the present disclosure provides a composition for targeted alteration of a DNA sequence and methods of altering the targeted DNA sequence using the composition. In some embodiments such a composition comprises a MiniVector comprising a nucleic acid sequence template for homology-directed repair, alteration, or replacement of the targeted DNA sequence within a cell in vivo or in vitro, where the MiniVector lacks both a bacterial origin of replication and an antibiotic selection gene, and wherein the Mini Vector has a size up to about 2,500 base pairs.
