A novel composition comprising Calcium chloride, Sodium Stearate and Sodium Hydroxide dissolved in water (pH of 9-11). The composition is used for obtaining isolated protein from seeds and commercially available de-oiled cake oilseeds /crop plant seeds the steps of incubation with novel composition solvent, filtration, purification, neutralization and precipitation. The precipitate is further washed, neutralized and dried to obtain the protein isolates. The specially designed solvent has a pH of 9-11 and comprises of calcium chloride, Sodium Stearate and Sodium Hydroxide dissolved in water. The calcium chloride percentage varies from 0.5-5%, Sodium Stearate 0.05-2% and sodium hydroxide to maintain the pH 9-11.
