The eye applicator for injection application of substance, especially of medical or diagnostic liquid substance or liquid to refraction alter, into eye tissue, especially in corneal tissue. The applicator comprises of the hollow main body, in which there is directly or by means of a segment one or more injectors embedded, especially one or more application needles. The main body can well be of the shape of a cone or cylindrical ring with at least one side cutout, together with the fact that there is a segment in the form of a hollow body with a lateral area corresponding to the hollow formatively and with at least one frontal base with an opening. The segment is rotatively embedded in the hollow of the main body. Inside the segment there are at least two movingly embedded replaceable stirrup needle holders holding on the syringe needles on their curved frontal areas and being equipped with thumbs nesting in curved grooves on the inner side of the segment base. The main body of the applicator can be also of the shape of a disc, which is directly equipped with openings for the needle insertion.