Федеральное государственное бюджетное учреждение науки Институт проблем экологии и эволюции им. А.Н. Северцова Академии Наук (RU)
发明人:
Ушакова Нина Александровна (RU),Павлов Дмитрий Сергеевич (RU),Стоянова Лидия Григорьевна (RU)
申请号：
RU2013125554/10
公开号：
RU2013125554A
申请日:
2013.06.03
申请国别(地区):
RU
年份:
2014
代理人:
摘要：
1. A method of obtaining probiotic preparation feed for farm animals, characterized in that the preparation is carried out by mixing fitonositelya 4-4.5 kg vegetable cellulose substrate with 1 liter of tap water, 150-160 grams of bakers yeast autolysate, 3.2 g of potassium disubstituted fosfornokislovo , 0,125-0,130 g of magnesium sulfate, 1.5-1.8 g of molasses and autoclave sterilization medium to make the resulting liquid culture fitonositel Cellulomonas flavigena VKPM B-2559 and incubated at 45-47 ° C for 4-4.5 h and then make a liquid Culture Bacilis subtilis VKPM B-8130 and Lactococcus lactis subsp.lactis VKPM B-4591 in equal amounts, the resulting mixture was stirred and maintained at 37-38 ° C for 48-50 hours and dried to a moisture content of 8-10% .2. A method according to claim 1, characterized in that Bacilis subtilis VKPM B-8130 grown on a medium containing, wt.%: Glucose 2.0-2.2, 0.5-0.6 peptone, yeast extract 0,3-0 4, 0.1-0.12 dibasic potassium phosphate, potassium dihydrogen phosphate 0,05-0,055, magnesium sulphate 0,02-0,022, tap water - ostalnoe.3. A method according to claim 1, characterized in that the Cellulomonas flavigena VKPM B-2559 grown on a medium containing, wt.%: Glucose 2.0-2.2, 0.5-0.6 peptone, yeast extract 0.3 0.4, potassium phosphate dibasic, 0.1-0.12, 0,05-0,055 monobasic potassium phosphate, magnesium sulfate 0,02-0,022, chopped straw 25-27, tap water - ostalnoe.4. A method according to claim 1, characterized in that the Lactococcus lactis subsp.lactis VKPM B-4591 grown on a medium containing, wt.%: 2.0-2.2 sucrose, potassium phosphate monobasic 0,2-0,22, magnesium sulphate 0,02-0,022, bakers yeast autolysate mg 35-37% of the ammonium nitrogen tap water - ostalnoe.5. A method according to claim 1, wherein Th1. Способ получения кормового пробиотического препарата для сельскохозяйственных животных, характеризующийся тем, что проводят приготовление фитоносителя путем смешивания 4-4,5 кг клетчаткового растительного субстрата с 1 л водо
