A photodynamic therapy and diagnosis device capable of optical fiber puncturing, comprising an optical fiber, a laser device (14), a light splitting coupler (12) and a fluorescence analyzer (13). The optical fiber comprises a body portion (8) and a puncture needle, one end of the body portion (8) is connected to the puncture needle, and the other end thereof is connected to the light splitting coupler (12). The light splitting coupler (12) is connected to both of the laser device (14) and the fluorescence analyzer (13) to enable a laser in the laser device (14) to enter the optical fiber through the light splitting coupler (12), and to enable fluorescence generated by a photosensitizer after absorbing the laser to enter the fluorescence analyzer (13) through the optical fiber and the light splitting coupler (12). In the therapy and diagnosis device, a laser having a wavelength allowing absorption by a photosensitizer drug is emitted by the laser device (14), passes through the light splitting coupler (12), and then reaches a tumor site in a body through an optical fiber puncture needle tube (10), and red light is absorbed by the photosensitive drug in the tumor and kills tumor cells. Upon excitation, the photosensitive drug generates fluorescence, which then enters the fluorescence analyzer (13) through the light splitting coupler (12), and the fluorescence quantum yield, the therapy effect, etc. can be analyzed by means of the analysis result, thereby achieving the effects of synchronous therapy and diagnosis.L'invention concerne un dispositif de thérapie et de diagnostic photodynamiques aptes à une ponction par fibre optique, comprenant une fibre optique, un dispositif laser (14), un coupleur à division de lumière (12) et un analyseur de fluorescence (13). La fibre optique comprend une partie corps (8) et une aiguille de ponction, une extrémité de la partie corps (8) étant reliée à l'aiguille de ponction, et l'autre extrémité au coupleur à division de lumière (12)
