The invention provides an aquatic animal feed, which comprises a carrier, a film layer and a plurality of probiotics. The probiotics can be gathered on the surface of the carrier to form a film layer covering the whole carrier under the condition of room temperature by using the microalgae which can supply water for living animals to eat and has palatability and suspension as the carrier, and can retain the original activity of the probiotics, so as to form a film layer covering the whole carrier. At the same time of feeding into the carrier, the Fed object can supplement nutrition, and ingest a large number of probiotics.本發明提供一種水生動物飼料,包含有一載體、一薄膜層以及複數益生菌,係利用可供水生動物食用,且具有適口性及懸浮性的微藻作為載體,在室溫的條件下,讓益生菌大量聚集附著於在載體表面,形成包覆整個載體的薄膜層,而能保有益生菌原有的活性,使得被餵食的對象在吃進載體的同時,能夠補充營養,並且攝取到大量的益生菌。