Aris N. Economides,David M. Valenzuela,Samuel Davis,George Yancopoulos
申请号：
US16116647
公开号：
US20180371493A1
申请日:
2018.08.29
申请国别(地区):
US
年份:
2018
代理人:
摘要：
Methods of creating mutations in genomic exons by inserting introns into the genomic exons via homologous recombination. Also, methods are provided for introducing modifications into genomic exons by inserting introns into the genomic exons via homologous recombination such that a mature mRNA transcript produced from a genomic region of the genome comprising the genomic exon does not contain the modification are provided. The methods provide for a rapid method for introducing mutations and/or modifications of any type into a mammalian cell genome.
