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Method for inducing vascular endothelial cells
专利权人:
Kyoto University;Osaka University
发明人:
Tatsutoshi Nakahata,Megumu Saito,Akira Niwa,Ryo Ota,Kiyotoshi Sekiguchi
申请号:
US15745425
公开号:
US10669529B2
申请日:
2016.07.14
申请国别(地区):
US
年份:
2020
代理人:
摘要:
Provided is a method for producing vascular endothelial cells from pluripotent stem cells, the method comprising the following steps (i) to (iii): (i) a step of culturing pluripotent stem cells in a culture medium comprising a BMP, on a culture vessel coated with a first matrix, to produce mesodermal progenitor cells; (ii) a step of dissociating the resulting cells into single cells; and (iii) a step of culturing the resulting cells in a culture medium comprising VEGF, on a culture vessel coated with a second matrix selected from the group consisting of laminin-411 or a fragment thereof, laminin-511 or a fragment thereof, Matrigel, type IV collagen and fibronectin.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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