Уласов Алексей Валентинович (RU);Розенкранц Андрей Александрович (RU);Соболев Александр Сергеевич (RU);Сластникова Татьяна Александровна (RU)
发明人:
Розенкранц Андрей Александрович (RU),Сластникова Татьяна Александровна (RU),Уласов Алексей Валентинович (RU),Соболев Александр Сергеевич (RU)
申请号：
RU2016123920
公开号：
RU2016123920A
申请日:
2016.06.16
申请国别(地区):
RU
年份:
2017
代理人:
摘要：
FIELD: pharmacy.SUBSTANCE: group of inventions relates to a composition for attaching a radioisotope to a polypeptide with a covalently attached chelator and to a method of attaching a radioisotope to a polypeptide with a covalently attached chelator. Composition consists of polypeptide and attached radionuclide, sodium citrate, 4-(2-hydroxyethyl)piperazine-1-ethanesulphonic acid (HEPES), sodium chloride, sodium dodecyl sulphate (SDS) with pH value selected for attachment of radioisotope, which is in range of 4–7.5 units. In the method, a radioisotope is added to a polypeptide with a joined chelator and incubated in a medium containing sodium citrate in range of 1–20 mM, 4-(2-hydroxyethyl)piperazine-1-ethanesulphonic acid (HEPES) concentration of 80 mM, sodium chloride, sodium dodecyl sulphate (SDS) in concentration of 0.02 %, at temperature of 37 °C, at pH in range of 4–7.5 units, after which sodium chloride is added to the physiological concentration of ions in the mixture and sodium hydroxide to pH 7.5.EFFECT: group of inventions provides a composition which enables to attach radioisotopes which readily form insoluble hydroxides at pH greater than 3–3,5, to polypeptides which are poorly soluble in a weakly acidic medium (pH 3–6).18 cl, 17 dwg, 19 ex
