WATKINS, Michael I.,MARR, Gregory A.,YANG, Xiaoyun,BRUEHL, Richard,SHAN, Daming,COLEMAN, Patrick F.
申请号：
AU2013208412
公开号：
AU2013208412A1
申请日:
2013.07.22
申请国别(地区):
AU
年份:
2013
代理人:
摘要：
#$%^&*AU2013208412A120130822.pdf#####Abstract The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase. Fig. 1
