Bruehl, Richard,Marr, Gregory A.,Shan, Daming,Watkins, Michael I.,Yang, Xiaoyun,Coleman, Patrick F.
申请号：
AU2009239558
公开号：
AU2009239558B2
申请日:
2009.04.17
申请国别(地区):
AU
年份:
2013
代理人:
摘要：
The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.
