The present invention relates to biomarkers, methods and assay kits for predicting prognosis and/or monitoring progression of prostate cancer. The biomarkers include a glycosyltransferases [core 1 beta-3-galactosyltransferase (C1GALT1) and/or ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3GAL1)] gene product, its saccharide substrate/product, and/or a galectin-4 gene product.本發明係有關預測前列腺癌預後及/或監控前列腺癌惡化之生物標記、方法、及試驗套組。生物標記包括醣基轉移酶[核心1 β-3-半乳糖基轉移酶(C1GALT1)及/或ST3 β-半乳糖苷α-2,3-唾液酸基轉移酶1 (ST3GAL1)]基因產物、其醣受質/產物、及/或半乳糖凝集素-4基因產物。