The present invention relates to a new non-toxigenic strain of Aspergillus flavus, that has been isolated in Italy and assigned to VCG IT6, and also to compositions comprising the same, and to the use thereof as a biocontrol agent against the contamination by aflatoxins produced by toxigenic strains of Aspergillus flavus and/or Aspergillus parasiticus. In addition, the present invention also provides a method for preventing and/or reducing and/or eliminating the contamination by aflatoxins produced by toxigenic strains of Aspergillus flavus and/or Aspergillus parasiticus in the main crops that can host the strain.