PROBLEM TO BE SOLVED: To provide a piercing device allowing smooth and certain collection and transfer of a membranous cell culture.SOLUTION: This piercing device 10 includes: a plurality of piercing members 14; and guide parts 15 guiding the plurality of piercing members movably forward and backward. The plurality of piercing members project from the guide parts such that tip parts of the plurality of piercing members move away from each other and move forward, and move backward such that the tip parts of the plurality of piercing members gather together.【課題】膜状細胞培養物の回収及び移送を円滑且つ確実に行い得る突刺デバイスを提供する。【解決手段】突刺デバイス10は、複数の突刺部材14と、複数の突刺部材を進退可能に案内する案内部15と、を有し、複数の突刺部材は、複数の突刺部材の先端部が互いに離間するように案内部から突出して前進するとともに、複数の突刺部材の先端部が互いに集まるように後退する。【選択図】図1