Disclosed herein are high Tm RNA nanostructures that can be composed of one or more modules or motifs to build RNA nanostructures with or without layers. The RNA nanostructures can have a core domain and three or more double-stranded arms and formulations thereof to conjugate high copy numbers of therapeutics, pH responsive or enzyme cleavable drug cargo. Also described herein is a design strategy for generation of synthetic RNA oligonucleotides that can self assemble into highly thermostable RNA structures. Also described herein are uses of the RNA nanostructures described herein.本文公開的是高Tm RNA奈米結構，其可以由用以構建分層或不分層的RNA奈米結構的一個或多個模塊或基序組成。所述RNA奈米結構可具有核心結構域和三個或更多個雙鏈臂及其製劑以綴合大量的治療劑、pH響應或酶可裂解的藥物貨物。本文還公開用於生成可自組裝成高度熱穩定的RNA結構的合成RNA寡核苷酸的設計策略。本文還公開本文所述的RNA奈米結構的用途。
