There is provided a sterilization apparatus capable of delaying degradation of sterilant in a cartridge or a tank in which an extraction tube is inserted. A sterilization apparatus extracting sterilant from a cartridge containing the sterilant and sterilizing a sterilization target includes an extraction tube configured to extract the sterilant from the cartridge, and a movement unit configured to move the extraction tube so that a position of the extraction tube with respect to the cartridge in a case of extracting the sterilant in the cartridge by the extraction tube and a position of the extraction tube with respect to the cartridge in a case of waiting for extraction of the sterilant from the cartridge by the extraction tube to perform following sterilization processing with the same cartridge may differ from each other.