JANG, MYUNG HYUN,張明賢,KIM, MIN YOUNG,金玟永,LEE, JONG-SOO,李鐘守,KIM, DAE JIN,金大振,BAE, SUNG MIN,裴城敏,KWON, SE CHANG,權世昌
申请号：
HK15102405.8
公开号：
HK1201858A
申请日:
2015.03.10
申请国别(地区):
HK
年份:
2015
代理人:
摘要：
Disclosed is a method for the preparation of a complex in which a physiologically active polypeptide is covalently bonded to an immunoglobulin constant region via a non-peptidyl linker. The method is characterized by the employment of a reducing agent, by which conventional problems of low production yield and modification of the polypeptide can be overcome. The physiologically active polypeptide-non-peptidyl polymer-immunoglobulin constant region complex can be produced with high purity and yield as well as at low cost. Thus, the method is industrially useful. Moreover, by exhibiting a prolonged action profile, the physiologically active polypeptide-non-peptidyl polymer-immunoglobulin constant region complex can be effectively used for developing long-acting formulations of physiologically active polypeptides which have improved drug compliance.
