LAKSHMI SASTRY-DENT,MATTHEW SIMPSON,STEVEN R. WEBB,ZEHUI CAO,WEI CHEN,NING ZHOU
申请号：
ARP130104696
公开号：
AR093979A1
申请日:
2013.12.13
申请国别(地区):
AR
年份:
2015
代理人:
摘要：
1. This note provides various methods to detect and determine plant events with Loki (location or location) in precisely selected genomes, as well as plants and cells with these selected genomes. This method can be used as a high-performance process to select intact conditions, or to select and selectively dislocate genomic chromosomes, so as to detect the position of DNA polynucleotide in the preferred genomic attributes.You. These methods can easily be applied to the methods of determining plant events by using the specific location nucleon system. Requirement 1: a method for determining the presence of foreign donor DNA polynucleotide inserted into the target genomic endpoint or pointIt includes: (a) expanding genomic DNA samples in the first amplification reaction, including objective genomic pronunciation, using the first oligonucleotide complex combined in a sterile manner near the pronunciation point of the target genome, Therefore, the first microphone can be generated, including the target's genome pronunciation； and (b) the presence or absence of the first microphone is found, because the absence of the first microphone indicates the presence of the donor's polynucleotide in the target's genome pronunciation.La presente descripción proporciona métodos para detectar e identificar eventos de plantas que contienen loci (lugares o sitios) genómicos elegidos con precisión, y plantas y células de plantas que comprenden tales loci genómicos elegidos. El método puede llevarse a cabo como un proceso de alto rendimiento utilizado para seleccionar la condición intacta o la disrupción de un loci genómico elegidos y opcionalmente para detectar una inserción de polinucleótidos de ADN donante en los loci genómicos elegidos. Los métodos son fácilmente aplicables para la identificación de eventos de plantas producidos vía un método de determinación que resulta del uso de una nucleasa específica de sitio. Reivindicación 1: Un método para identificar la presencia de un polin
