PURPOSE: A composition containing pseurotin D for preventing and treating cancer is provided to suppress cell growth due to apoptosis and DNA damage. CONSTITUTION: A pharmaceutical composition for preventing and treating cancer contains 0.02-50 weight% pseurotin D of chemical formula 1 as an active ingredient. A health food for preventing and treating cancer contains pseurotin D as an active ingredient. The health food is a tablet, capsule, pill, or liquid. A method for isolating pseurotin D comprises: a step of culturing brown algae of Aspergillus fumigatus in a SWS medium and filtering to isolate mycelium a step of isolating the culture liquid with EtOAc and performing silica gel column chromatography and a step of purifying a fraction containing pseurotin D by MPLC(medium pressure liquid chromatogrhy) and HPLC.본 발명은 암질환 예방 및 치료용 조성물에 관한 것으로, 보다 상세하게는 푸슈로틴(pseurotin) D를 유효성분으로 함유하는 암 질환의 예방 및 치료용 조성물에 관한 것이다. 본 발명의 조성물은 세포사멸(apoptosis)의 유도와 DNA 손상에 의한 세포성장의 저해를 나타냄으로서, 암 질환의 예방 및 치료에 유용하게 사용될 수 있다.