PROBLEM TO BE SOLVED: To provide a biological information acquisition device capable of measuring a pulse wave with a high degree of precision.SOLUTION: A biological information acquisition device 1 includes: a first detection part 11 for irradiating light of a first wavelength to a living body and detecting reflected light or transmitted light in the living body a second detection part 12 for irradiating light of a second wavelength to the living body and detecting reflected light or transmitted light in the living body and a processing part (e.g., a processing device 30) for deriving a pulse wave of the living body based on a subtraction value that subtracts a detection signal acquired by irradiating the light of the second wavelength to the living body from a detection signal acquired by irradiating the light of the first wavelength to the living body.SELECTED DRAWING: Figure 1COPYRIGHT: (C)2016,JPO&INPIT