The present invention provides a composition containing Dendrobium candidum extract and a preparation method and use thereof. The composition containing Dendrobium candidum extract comprises the following raw materials in parts by mass: 4-6 parts of sodium hyaluronate, 0.5-1.5 parts of Dendrobium candidum stem extract, 0.5-1.5 parts of Crocus sativus extract, 0.5-1.5 parts of Cereus grandiflorus extract, 0.5-1.5 parts of Centaurea cyanus extract and 0.5-1.5 parts of Scutellaria baicalensis root extract. The composition containing Dendrobium candidum extract prepared by the present invention has a good moisturizing effect and can effectively repair skin epidermal cells. The preparation process is simple. Identification and risk assessment were carried out on the safety risk substances of the prepared facial mask, and no safety risk was found in the use of the product.