Peptide with 13 points of 56 minutes residues discloses a kind of with biological activity. These peptides account for peptide HB-107 (SEQ short fragment sequence numbers: 1), the antibacterial protein cecropin B of itself segment, and put on display stimulation cell, migration and anti-inflammatory property. When keratinocyte is especially sensitive with this meaning, disclosed peptide includes beneficial agents medical injury skin, from such as diabetic ulcer. The peptide also effectively prevents and reversing skin surface damage is due to various environments. Importantly, therapeutic effect is obvious, the concentration of peptide is equal to or more than those peptides HB-107, and therefore indicates the method exploitation effectively treatment that a tiny bit is expensive, more general. The preparation and application of these peptides is also disclosed..