LEE, Byeong Chun,이병천,HONG, Hye Jin,홍혜진,AHN, Cu Rie,안규리,KIM, Geon A,김건아,YEOM, Su Cheong,염수청,KIM, Su Jin,김수진,CHO, Bum Rae,조범래,LEE, Eun Mi,이은미,LEE, Sang Hoon,이상훈,HWANG, In Chang,황인창
申请号：
KRKR2016/012993
公开号：
WO2017/082667A1
申请日:
2016.11.11
申请国别(地区):
WO
年份:
2017
代理人:
摘要：
The present invention relates to a transgenic pig which simultaneously expresses a heme oxygenase-1 (HO-1) gene and a tumor necrosis factor receptor 1-Fc (TNFR1-Fc) gene and comprises a knocked-out α-1,3-galactosyltransferase (GGTA1) gene and in which an immune rejection response is inhibited upon xenotransplantation, and a method for preparing the same. The transgenic pig which simultaneously expresses human HO-1 and TNFR1-Fc fusion genes and comprises a knocked-out GGTA1 gene, according to the present invention, can reduce oxidative stress upon organ separation and in vitro culturing by the antioxidative action, cell protection functions, etc. of the HO-1 gene, can reduce a TNF-α mediated inflammatory response occurring during an initial transplant period by the expression of TNFR1-Fc, and also can inhibit maturation of dendritic cells and control the proliferation and activation of T-cells to reduce acute vascular rejection, thereby improving the initial engraftment of the transplanted organ. Furthermore, a hyperacute immune rejection is inhibited by GGTA1 whereby the survival rate of the transplanted organ is increased. Therefore, it is possible to produce an organ in which an immune rejection response is inhibited upon heterograft using the transgenic pig.La présente invention concerne un porc transgénique qui exprime simultanément un gène dhème oxygénase-1 (HO-1) et un gène de récepteur de facteur de nécrose tumorale 1-Fc (TNFR1-Fc), et comprend un gène de α-1,3-galactosyltransférase (GGTA1) inactivé et dans lequel une réponse de rejet immunitaire est inhibée lors dune xénogreffe, et son procédé de préparation. Le porc transgénique qui exprime simultanément des gènes de fusion HO-1 et TNFR1-Fc humains et comprend un gène GGTA1 inactivé, selon la présente invention, peut réduire le stress oxydatif lors dune séparation dorgane et dune culture in vitro par laction antioxydante, des fonctions de protection cellulaire, etc., du gène HO-1, peut réduire une réponse i
