WU, YANG CHANG,吴永昌,吳永昌,LU, MEI CHIN,吕美津,呂美津,CHANG, FANG RONG,张芳荣,張芳榮,DU, YING CHI,杜英齐,杜英齊,WU, TUNG YING,吴东颖,吳東穎
申请号：
TW098104772
公开号：
TWI389699B
申请日:
2009.02.13
申请国别(地区):
TW
年份:
2013
代理人:
摘要：
A preparation method for an ethanol extract of fruiting bodies ofAntrodia camphorata(EEAC) is provided. The preparation method includes steps of: (a) providing the fruiting bodies ofA. camphorata, and (b) extracting, filtering and centrifuging the fruiting bodies ofA. camphoratausing an ethanol solution, so as to obtain the EEAC. EEAC further can be sequentially extracted or fractioned byn-hexane, ethyl acetate and ethanol, and ann-hexane fraction (FC), an ethyl acetate fraction (FA) and an ethanol fraction (FB) respectively are generated. The growth inhibition and apoptosis induction of Leukemia cell line HL 60 are effectively mediated by the FA product, in which zhankuic acid A is the bioactive marker. The amount of zhankuic acid A inA. camphoratafruiting bodies can be determined by nuclear magnetic resonance and high performance liquid chromatography analysis.本案係提供一種樟芝子實體乙醇萃取物的製備方法,包括下列步驟:(a)提供一樟芝子實體;及(b)以一乙醇溶液萃取該樟芝子實體,過濾並離心,獲得該樟芝子實體第一乙醇萃取物。該樟芝子實體第一乙醇萃取物還可以正己烷、乙酸乙酯及乙醇依序萃取或分餾,分別獲得樟芝子實體正己烷、乙酸乙酯萃取物及第二乙醇萃取物。樟芝子實體第一乙醇萃取物可有效抑制血癌細胞生長並促進細胞凋亡,樟芝子實體乙酸乙酯萃取物內含活性主成分-樟芝酸A,可做為生物活性指標。以核磁共振圖譜及高效液相層析分析法可檢測樟芝子實體的樟芝酸A含量。101‧‧‧樟芝子實體102‧‧‧以乙醇溶液萃取103‧‧‧樟芝子實體第一乙醇萃取物(EEAC)104‧‧‧樟芝子實體正己烷萃取物(FC)105‧‧‧第一樟芝子實體殘留物106‧‧‧樟芝子實體乙酸乙酯萃取物(FA)107‧‧‧第二樟芝子實體殘留物108‧‧‧樟芝子實體第二乙醇萃取物(FB)109‧‧‧第三樟芝子實體殘留物110‧‧‧樟芝子實體乙醇萃取殘留物111‧‧‧以水萃取112‧‧‧樟芝子實體水萃取物(WEAC)
