The invention provides a biosurfactant produced from microorganism Bacilus subtilis-surfactin. The surfactin is natural ring-type lipopeptid with an annular structure formed from seven amino acids the surfactin has the functions of anti-aging, anti-wrinkle, skin-penetrating power for cosmetic (permeation-promoter), foaming agent, emulsifying agent, etc.一種由微生物Bacillus subtilus所生產之生物介面活性劑biosurfactant一表面素(surfactin),該表面素是一個天然環型的脂胜肽,由七個胺基酸形成一個環狀的結構;且該表面素具有皮膚抗老化、抗皺、增加化妝品皮膚穿透力(助滲劑)、起泡劑及乳化劑等功能。