The invention discloses an electron beam irradiation disinfection apparatus for film material. On the inlet (10A) and the outlet (10B) of the film material disposed in an irradiation treatment chamber(10) in pressure reduced state, pressure reduction holding and adjusting devices (21), (22) are mounted by sealing mode each of the pressure reduction holding and adjusting devices (21), (22) is equipped with a pair of clamping frame (24) in the frame body (23) in order to press and clamp the film material (1) on side of the clamping frame (24) is set as fixed side the other side is set as movable side which can move back and forth in the frame body (23) by a pressing moving apparatus the clamping frame (24) is composed of a plurality of continuous sub-frames (25) and small caliber rollers (26) respectively disposed in every sub-frame. The invention can hold the pressure reduced state well in the irradiation treatment chamber, and can be miniaturized for being made economically.