Exemplary embodiments are directed to instruments, methods and systems for harvesting and implanting graft materials, including instruments for capturing a surface topography of an anatomical location, instruments for defining an implant region, and graft harvesting devices. Exemplary embodiments are also directed to methods for capturing a surface topography of an anatomical location, methods for defining an implant region, and methods for harvesting a donor plug. The exemplary instruments, methods and systems generally include capturing a surface topography of a defect region, creating a defect region cavity, and harvesting a donor plug configured and dimensioned to be implanted in the defect region cavity.