AKULA, ANISHA,DUNCAN, DAVID R.,LOWE, BRENDA,MANN, MICHAEL T.,PETERSEN, WILLIAM L.,ROUT, JYOTI R.,SONGSTAD, DAVID D.,WILKS, JOEL B.,ZHANG, WANGGEN
申请号：
CA2884022
公开号：
CA2884022C
申请日:
2007.08.31
申请国别(地区):
CA
年份:
2017
代理人:
摘要：
A method for producing a transformable and embryogenic corn cell culture comprising: a) obtaining a Type II callus from corn embryos； b) initiating a cell suspension culture from cells of the callus； and c) culturing the cell suspension for about 5 days to 30 days in a liquid medium to produce the cell culture, wherein the cell culture is transferred to fresh growth media every 2-3 days； and wherein undesirable cell types are removed from the cell culture during transfer to fresh growth media.
