Общество с ограниченной ответственностью "Институт биотехнологий и фармакологии"; ООО "ИНБИОФАРМА" (RU)
发明人:
Волчков Станислав Евгеньевич (RU),Тюмина Ольга Владимировна (RU)
申请号：
RU2018119842
公开号：
RU2018119842A
申请日:
2018.05.29
申请国别(地区):
RU
年份:
2019
代理人:
摘要：
FIELD: medicine.SUBSTANCE: invention refers to medicine, and can be used for producing cytokines from umbilical blood thrombocytes. Method involves three stages: at the first stage, umbilical blood is sampled from different donors, Stabisol solution is administered in each packet with donor blood in amount of 20 % of the blood volume of the packet, each blood pack is centrifuged in a smooth program change mode, as a result of which blood is separated into thrombocyte enriched plasma and erythrocytes. Said program consists of the following modes: acceleration to 540 RCF for 5 minutes； acceleration to 900 RCF for 2 minutes； braking to 300 RCF and rotation for 2 minutes； braking up to 100 RCF and rotation for 1.3 minutes with subsequent disconnection of the centrifuge, then removing the erythrocytes from the packs and recycling them. At the second stage, all donor OTP packets are combined and all thrombocytes are concentrated, for this purpose the produced thrombocyte enriched plasma is transferred into the appropriate number of 50 ml-type falcon test tubes, centrifugation is carried out for concentration of thrombocytes in mode of 15 minutes at rate of 4000g, as a result of centrifugation a platelet concentrate and lean plasma are obtained. Oblong plasma is taken for use as a solvent, leaving 5 ml of plasma at the bottom of the test tubes, obtained thrombocyte concentrates of different donors are combined in one test tube with volume of 50 ml and diluted with depleted plasma to volume 50 ml or the solvent used is normal saline, if further plasma proteins need to be excluded； obtained thrombocyte concentrate pool is re-centrifuged in 60-minute mode at rate of 50g for removal of erythrocyte and leukocyte residues. After centrifugation, the supernatant fluid in amount of 45–48 ml is transferred into new 50 ml test tube, and 1 ml of the solution is sent to a laboratory for platelet count on an automated haematological analyzer. At the third stage, the obtained thrombocyte
