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METHODS OF MODIFYING GENES IN EUKARYOTIC CELLS
专利权人:
REGENERON PHARMACEUTICALS, INC.
发明人:
Economides Aris N.,Valenzuela David M.,Davis Samuel,Yancopoulos George
申请号:
US201816116647
公开号:
US2018371493(A1)
申请日:
2018.08.29
申请国别(地区):
美国
年份:
2018
代理人:
摘要:
Methods of creating mutations in genomic exons by inserting introns into the genomic exons via homologous recombination. Also, methods are provided for introducing modifications into genomic exons by inserting introns into the genomic exons via homologous recombination such that a mature mRNA transcript produced from a genomic region of the genome comprising the genomic exon does not contain the modification are provided. The methods provide for a rapid method for introducing mutations and/or modifications of any type into a mammalian cell genome.
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