PROBLEM TO BE SOLVED: To provide a method for preparing a lactic acid bacterium starter capable of exhibiting high fermentation activity, and a method for producing fermented milk using a highly active lactic acid bacterium starter prepared in this way.SOLUTION: In the method for preparing the highly active lactic acid bacterium starter, cultivation of Bacillus bacteria and Thermophilus bacteria planted to coexist with each other in a skim milk culture medium containing 10% (W/W) or more of a skim milk component and 0.2% (W/W) or more of an yeast extract is carried out in a neutralized condition by controlling pH to 5-6. The highly active lactic acid bacterium starter prepared in this way and the method for producing the fermented milk using the lactic acid bacterium starter are also provided.COPYRIGHT: (C)2012,JPO&INPIT【課題】高い発酵活性を発揮できる乳酸菌スターターを調製する方法と、これにより調製した高活性の乳酸菌スターターを使用した発酵乳の製造方法を提案する。【解決手段】脱脂乳成分を10%(W/W)以上、酵母エキスを0.2%(W/W)以上で含む脱脂乳培地に、ブルガリア菌とサーモフィラス菌を共存させて植え付けて、pHを5~6に制御して中和培養する高活性の乳酸菌スターターの調製方法。これによって調製した高活性の乳酸菌スターターと、これを使用した発酵乳の製造方法。【選択図】なし