An analytical test strip for the determination of an analyte (such as glucose and/or hematocrit) in a bodily fluid sample (such as a whole blood sample) includes a first capillary sample-receiving chamber, a second capillary sample-receiving chamber, and a physical barrier island disposed between the first and second capillary sample-receiving chambers. Moreover, the physical island barrier is disposed such that bodily fluid sample flow between the first capillary sample-receiving chamber and the second capillary sample-receiving chamber is prevented during use of the analytical test strip.一種用於測定一體液試樣(如一全血樣本)中之一分析物(如葡萄糖及/或血容比)之分析測試條,其包括一第一毛細管試樣接受室、一第二毛細管試樣接受室,及一經配置於該第一及第二毛細管試樣接受室之間的實體障壁島。另外,該實體障壁島配置為能夠在該分析測試條使用期間,防止體液試樣在該第一毛細管試樣接受室與該第二毛細管試樣接受室之間流動。100‧‧‧電化學式分析測試條120‧‧‧電絕緣基材層140‧‧‧圖案化導體層160‧‧‧圖案化絕緣層180‧‧‧電極暴露窗200‧‧‧酶試劑層220‧‧‧圖案化間隔物層220a‧‧‧實體障壁島240‧‧‧親水性層260‧‧‧頂層