An objective method and system for dynamic analysis of prosthesis- bound subjects for determining optimal prosthesis alignment adjustments consists of a motion detection system, motion database, blending engine and algorithms, and a graphical user interface with suitable program controls whereby manual, semi-automatic, or fully automated analysis of a prosthesis-equipped subject's motion performance can be done in real time to determine objectively the optimal adjustments for the subject's prosthesis in a precise clinical protocol context.