A device and a method for preparing adipose tissue for transplantation from lobular fat extracted for instance by liposuction said fat consisting of a fluid component comprising an oily component a blood component and/or sterile solutions and a solid component comprising cell fragments cells and one or more cell macroagglomerates of heterogeneous size wherein said device is composed of at least one washing and separating container (1) having a washing chamber (101) which container (1) has an inlet (102) and an outlet (103) for the liposuctioned material to enter the washing chamber (101) through the inlet (102) and for at least part of said material to exit said chamber (101) through the outlet (103) said washing chamber (101) including stirring means for forming an emulsion of fluid components.