A device and a method for preparing adipose tissue for transplantation from lobular fat extracted, for instance by liposuction, said fat consisting of a fluid component comprising an oily component, a blood component and/or sterile solutions and a solid component comprising cell fragments, cells and one or more cell macroagglomerates of heterogeneous size, wherein said device is composed of at least one washing and separating container (1) having a washing chamber (101) for washing the liposuctioned material, which container (1) has an inlet (102) and an outlet (103) for the liposuctioned material to enter the washing chamber (101) through the inlet (102) and for at least part of said material, particularly the fluid component, to exit said chamber (101) through the outlet (103), said washing chamber (101) including means for forming an emulsion of fluid components. A method of treating body and face volume deficiencies, improving skin trophism and/or for biological stimulation by the adipose tissue obtained through said device and method, which tissue is suitable for use as a source of cells such as chondrocytes, osteocytes, adipocytes.
