The present invention provides a fat burning ferment, preparation method thereof and use thereof for fat burning and losing weight. The fat burning ferment can effectively promote the decomposition of fat in fat cells, to reduce the gene expression level of PLIN1 and PPARG2 in fat cells to promote the decomposition of fatty oil droplets, and to promote the conversion of white fat cells to brown fat cells, which increases the ability to burn fat. The fat burning ferment is prepared by fermenting the apple, the ginger, the chili, and a cinnamon to yeast, lactic acid bacteria, and acetic acid bacteria for three-stage fermentation.本發明提供一種燃脂發酵物及其製備方法與用於製備燃脂減肥之組合物的用途，能有效促進脂肪細胞中脂肪的分解、亦能夠有效的降低脂肪細胞中PLIN1基因的表現量，促使脂肪油滴變得容易分解，並能同時有效提升PPARG2基因的表現量，促進白色脂肪細胞轉化為棕色脂肪細胞，使得身體燃燒脂肪的能力上升，其中該燃脂發酵物係將由一蘋果、一薑、一辣椒、及一肉桂所組成之混合物以酵母菌、乳酸桿菌、及醋酸桿菌依序進行三段式發酵而獲得。
