БАРНС Томас М. (US),ХОУ Цзиньчжао (US),ЗАРБИС-ПАПАСТОЙТСИС Грегори (US),ШИРМЕР Эмили Белчер (US),МАКНИЛ Гари Л. (US),ГОЛДЕН Кэтрин (US)
申请号：
RU2014107743/10
公开号：
RU2014107743A
申请日:
2012.07.27
申请国别(地区):
RU
年份:
2015
代理人:
摘要：
1. The purified preparation of a chimeric cytokine protein containing an amino acid sequence that is at least 90% identical to the sequence of SEQ ID NO: 21, where the chimeric cytokine protein is purified by more than 90%, where the preparation contains less than 10% of the des-Ala chimeric form cytokine protein, less than 10% of the acetylated form of the chimeric cytokine protein and less than 10% of the methionized form of the chimeric cytokine protein. 2. The purified preparation according to claim 1, wherein the purified preparation contains from 0.1 to 4% of the indicated form of the chimeric cytokine protein. The purified preparation according to claim 1, wherein the purified preparation contains from 0 to 5% of an acetylated form of a chimeric cytokine protein. The purified preparation according to claim 3, wherein the purified preparation further comprises from 0.1 to 4% of the methionized form of a chimeric cytokine protein. The purified preparation according to claim 1, wherein the purified preparation contains less than 8% of one or more forms of des-Ala chimeric cytokine protein, a methionized form of the chimeric cytokine protein and an acetylated form of the chimeric cytokine protein. A composition comprising a protein containing an amino acid sequence that is at least 90% identical to the sequence of SEQ ID NO: 21, salt and tonicity agent. The composition of claim 6, wherein the salt is sodium citrate, sodium acetate, sodium chloride, or Tris acetate. The composition of claim 6, further comprising a surfactant. The composition of claim 6, further comprising poloxamer 188, polysorbate 20, polysorbate 80, or polyethoxylate. The composition of claim 6, wherein the tonicity agent is sorbitol, mannitol, sucrose, trehalose, or glycerol. The composition of claim 6, wherein the pH is from 5.5 to1. Очищенный препарат химерного цитокинового белка, содержащего аминокислотную последовательность, которая по меньшей мере, на 90% идентична последовательности SEQ I
