Gabriel F.K. Everett,Charles Greenwald,Judy Pruitt,Amanda Rosmarin,Jordan Church,Daniel Aberle,George Aboagye
申请号：
US16785138
公开号：
US20200171104A1
申请日:
2020.02.07
申请国别(地区):
US
年份:
2020
代理人:
摘要：
A nutrient-germinant composition to aid in spore germination and a method for increased spore germination efficiency. The composition comprises L-amino acids, D-glucose and/or D-fructose, a phosphate buffer, an industrial preservative, and may include bacteria spores or they may be separately combined for germination. The method comprises providing a nutrient-germinant composition and bacteria spores, preferably of one or more Bacillus species, and heating to a preferred elevated temperature range of 41° C. to 44° C. for an incubation period of around 2 to 60 minutes. The nutrient-germinant composition is preferably in a concentrated liquid form that is diluted just prior to initiating the germination/incubation method at the point of use. The method may also include dispensing a germinated spore solution to a point-of-use/consumption, such as animal feed, water, or bedding, or a wastewater system or drain.
