This invention relates to a method of detecting or determining the presence of prostate cancer cells in a sample of body fluid from a subject comprising: (i) isolating cells from said sample to provide a cell sample； (ii) contacting said cell sample with a specific binding member capable of binding a prostate antigen； and/or (iii) contacting said cell sample with a specific binding member capable of binding a minichromosome maintenance (MCM) polypeptide(s)； and (iv) determining the binding of said specific binding member(s) to the cell sample.
