The invention provides a method of detecting a subject suffering from, or at risk of suffering from, bladder cancer the method comprisingi) providing a body fluid sample isolated from a subject；ii) isolating cells from said sample to provide a cell sample；iii) contacting the sample with a specific binding member capable of binding to a minichromosome maintenance (MCM) polypeptide(s)；iv) determining the binding of said specific binding member to the cell sample；v) counting those cells in said cell sample which bound to said specific binding member to provide a cell count；vi) determining, based on the cell count, whether the subject has, or is at risk of having, bladder cancer.
