The invention refers to a container (1) for collecting, transporting and storing biological tissue samples, wherein the container (1) is filled with a first component (3) being a fixative and a second component (4) being a fluid, wherein the second component (3) has a lower specific gravity compared to the fixative (3), and is immiscible with the fixative (3). The second component (4) is stratified on top of the fixative (3) to form a protective film to prevent fixative fumes to escape from the container (1). The invention further relates to a method for collecting, transporting and storing biological tissue samples. The method comprises the steps of: filling a first component (3) being a fixative in a container (1), and filling a second component (4) being a fluid in the container (1) filled with the first component (3), wherein the second component (4) has a lower specific gravity compared to the fixative (3) and is immiscible with the fixative (3) so that the second component (4) is stratified on top of the fixative (3) to form a protective film to prevent fixative fumes to escape from the container (1). The method further comprises the step of placing a biological tissue sample into the first component (3) before or after the second component (4) has been filled into the container (1).
