EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH;JEFFREY ALAN HUBBELL;UNIVERSITAET ZUERICH
发明人:
ANNA JEN,JASON SCHENSE,JEFFREY ALAN HUBBELL,MATTHIAS LUETOLF
申请号：
BR0215192
公开号：
BRPI0215192B1
申请日:
2002.12.18
申请国别(地区):
BR
年份:
2016
代理人:
摘要：
"Development factor-modified protein matrices for tissue construction". Proteins are incorporated into protein or polysaccharide matrices for use in tissue repair, regeneration and / or remodeling and / or drug release. proteins may be incorporated so that they are released by matrix degradation, diffusion and / or enzymatic action. As demonstrated by the examples, one method is to bind heparin to the matrix by either covalent or non-covalent methods to form a heparin matrix. heparin then non-covalently binds heparin binding development factors to the protein matrix. alternatively, a fusion protein may be constructed which contains a cross-linking region, such as a factor xiiia substrate and the natural protein sequence. The incorporation of degradable bonds between matrix and bioactive factors may be particularly useful when long-term drug release is desired, for example, in the case of nerve regeneration, where it is desirable to vary the drug release rate spatially as a function. regeneration, for example, slightly close to the living tissue interface and more slowly away within the injury zone. Additional benefits include the lower total drug dose in the delivery system, and spatial release adjustment that allows a larger percentage of the drug to be released at the time of increased cellular activity."matrizes de proteína modificadas pelo fator de desenvolvimento para construção do tecido". proteínas são incorporadas em matrizes de proteína ou polissacarídeo para uso em reparo, regeneração e/ou remodelagem de tecido e /ou liberação de droga. as proteínas podem ser incorporadas para que elas sejam liberadas por degradação da matriz, por difusão e/ou ação enzimática. como demonstrado pelos exemplos, um método é para ligar heparina à matriz por métodos ou covalentes ou não-covalentes, para formar uma matriz de heparina. a heparina, em seguida, liga não-covalentemente fatores de desenvolvimento de ligação de heparina à matriz de proteína. alternativamente, uma prot
