[Problem] The present invention provides a preparation jig for a tympanic membrane regenerating agent and a preparation vessel for a tympanic membrane regenerating agent enabling simple preparation of a tympanic membrane regenerating agent.[Solution] A preparation jig for a tympanic membrane regenerating agent (1a) and a preparation vessel for a tympanic membrane regenerating agent (1A) each include: a vessel (3A) including housing walls (6, 7) forming an interior space (S1) housing a medicinal solution support (2), and including an opening (6h) opening in one direction and formed in the housing walls (6, 7)； and a holding portion (4A) disposed in the interior space (S1). The deep side of the interior space (S1) is a housing portion (S3) housing the medicinal solution support (2) holding a medicinal solution. The opening (6h) side of the interior space (S1) is an installation space (S2) in which the holding portion (4A) is disposed.
