The present invention provides four purified preparation containing a polynucleic acid molecule encoding porcine Torque teno virus (PTTV) genotypes or subtypes PTTV1 a-VA, PTTV1 b-VA, PTTV2b-VA, and PTTV2c-VA. The present invention also provides infectious DNA clones, biologically functional plasmid or viral vector containing the infectious nucleic acid genome molecule of the same. The present invention further provides live, attenuated, vector-expressed and purified recombinant capsid subunit or killed viral vaccines for protection against PTTV infection. The present invention additionally provides subunit vaccines comprising PTTV specific gene products, especially ORF1 capsid gene product for protection against PTTV infection. Further, the present invention provides methods for diagnosing PTTV infection via polymerase chain reaction (PCR) using specific primer for PTTV1, PTTV2, and individual PTTV1 genotypes. Finally, the present invention provides methods for diagnosing PTTV infection via immunological methods, e.g., enzyme-linked immunoabsorbent assay (ELISA) and Western blot using PTTV specific antigens for detecting serum PTTV specific antibodies.Una vacuna que comprende un portador fisiológicamente aceptable, no toxico y una cantidad inmunogénica de una secuencia polipeptídica ORF1 de un virus Torque tena porcino (PTTV) 1a-VA completo, o un fragmento de subtipo inmunogénico específico. en donde la proteina ORF1 o fragmento inmunogénico de la proteína ORF1 comprende una región hipervariable (HVR) representada por los aminoácidos 382 a 460 de la secuencia SEO ID NO 14； Un método para diagnosticar infección por PTTV1b-VA； Una composición inmunogéníca que comprende una proteína representada por la secuencia de aminoácidos 322 a 639 de la secuencia SEO ID NO 14.
