The present invention provides methods of passage primate embryonic stem cells, a method for inducing differentiation into blood cells, such as vascular endothelial cells and the same cell. The present invention, cell detachment colonies of embryonic stem cells, vessel, no cytokine and under feeder-free, and cultured in a medium containing protein components, the resulting coated with the extracellular matrix, a primate embryonic stem cells the presence of cytokines, and cultured in free medium or serum-containing, was obtained, a process that includes the presence of the agent, were detached and seeded in medium of the same principal of the colonies, and the primate embryonic stem cells that the presence of cytokines, to obtain a specific progenitor cells in adherent culture, to obtain the vascular endothelial precursor cells and blood cells by separating the adherent cells and suspension cells from the particular progenitor cells and embryoid body-like cells aggregates or embryoid body , the method comprising.本発明は、霊長類動物胚性幹細胞の継代方法、同細胞の血管内皮細胞および血液細胞等への分化誘導方法を提供する。本発明は、霊長類動物胚性幹細胞を、細胞外マトリックスでコートされた容器中、無フィーダーおよび無サイトカイン下、蛋白成分を含有する培地で培養し、得られた胚性幹細胞のコロニーを細胞剥離剤の存在下、剥離し、該コロニーを同要の培地に播種することを含む方法、および霊長類動物胚性幹細胞を、サイトカインの存在下、血清含有または非含有培地で培養し、得られた胚葉体または胚葉体類似細胞凝集塊をサイトカインの存在下、接着培養して特定前駆細胞を得、該特定前駆細胞から浮遊細胞と接着細胞を分離して血液細胞および血管内皮前駆細胞を得ることを含む方法を提供する。
