The invention relates to a straw for cryopreservation that improves the chance of conception of semen after cryopreservation. The straw is produced by introducing a partitioning layer into a straw for artificial insemination to separate layers, thereby forming a semen storage layer containing an aqueous solution containing semen and an anti-freeze agent on one side and a dilution layer containing no anti-freeze agent on the other side, wherein a dilution solution containing an aqueous solution containing tris(hydroxymethyl aminomethane), citric acid, glucose and sodium chloride is used in the dilution layer.
