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IMPROVED CELL COMPOSITION AND METHODS FOR PRODUCING IT
专利权人:
АНТРОДЖЕНЕЗИС КОРПОРЕЙШН (US)
发明人:
ЗЕЙТЛИН Энди (US),РУССОТТИ Грегори (US),ХЭ Шуян (US),ПАЛ Аджай (US),ЧЭНЬ Хун Цз. (US),БРИВА Томас (US),ШОРР Райан (US),МЕРФИ Брайан (US)
申请号:
RU2011110455/13
公开号:
RU2011110455A
申请日:
2009.08.20
申请国别(地区):
RU
年份:
2012
代理人:
摘要:
1. A method of obtaining a composition containing cells, which includes:! (a) contacting said cells with a solution comprising dextran and human serum albumin (HSA) to obtain a solution containing the cells; ! (b) filtering the solution containing the cells; ! (c) if said cell-containing solution comprises more than about 10 ± 3 × 106 cells per milliliter, diluting said cells to not more than about 10 ± 3 × 106 cells per milliliter with a first dilution solution comprising dextran; and! (d) optionally diluting the solution containing the cells with a second dilution solution containing dextran but not containing HSA to obtain a composition containing the cells. ! 2. The method according to claim 1, where these cells undergo cryopreservation after stage (c) before stage (d). ! 3. The method according to claim 1, wherein said dextran in said first dilution solution or said second dilution solution is dextran 40.! 4. The method according to claim 1, wherein said dextran in said first dilution solution and said second dilution solution is dextran 40.! 5. The method according to claim 1, where the specified dextran in the specified solution of the first dilution is 5.5% dextran 40.! 6. The method according to claim 1, where the specified HSA in the specified solution, including HSA, is 10% HSA. ! 7. The method according to claim 1, where the specified solution of the first dilution includes HSA. ! 8. The method according to claim 7, where the specified HSA in the specified solution of the first dilution is 10% HSA. ! 9. The method according to claim 1, where the specified solution of the first dilution includes a cryoprotectant. ! 10. The method according to claim 9, where the specified cryoprotectant is a DMSO. !1. Способ получения композиции, содержащей клетки, который включает: ! (а) контактирование указанных клеток с раствором, включающим декстран и сывороточный альбумин человека (HSA), для получения раствора, содержащего клетки; ! (b) фильтрацию раствора, содержащ
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