A peptide for promoting gonad maturity, mating, and spawning of crustaceans, which has amino acid sequences of SEQ ID NO: 1 or SEQ ID NO: 2. The peptide of the present invention can promote the gonad maturity of the crustaceans effectively to elevate the quality of reproduction so as to promote the spawn quantity, spawn yield, and fertility of the animal, which makes the animals filial generation (nursery stock) healthier, the breeding production can beincreased, and physiological injury and side effect made by unilateral eyestalk abalation technique can be reduced.一種用以促進甲殼類動物生殖腺成熟、交配行為、產卵的胜肽,具有如SEQ ID NO:1或SEQ ID NO:2所示之胺基酸序列,本發明之胜肽可用以有效促進甲殼類生殖腺成熟,以提昇其生殖配子之品質藉以促進該動物產卵量、產卵率與繁殖力,使該動物的子代(種苗)更為健康,並增加養殖的產量;並能減少因剪眼柄(unilateral eyestalk abalation technique)催熟技術所造成之生理傷害與副作用。